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Description: In vitro matured bovine oocytes have been denuded of most cumulus cells by vortexing. Denuding allows visualization of the ooplasm for such procedures as microinjection of nuclei for cloning, gene constructs for transfection, and addition and removal of cryoprotective agents (CPA) for cryopreservation. Presence of a single polar body in the perivitelline space indicates that oocytes have progressed to metaphase II.
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