In vitro matured bovine oocytes have been denuded of most cumulus cells by vortexing.  Denuding allows visualization of the ooplasm for such procedures as microinjection of nuclei for cloning, gene constructs for transfection, and addition and removal of cryoprotective agents (CPA) for  cryopreservation.  Presence of a single polar body in the perivitelline space indicates that oocytes have progressed to metaphase II.

Denuded in vitro matured bovine oocytes

Credit: Sanjay Khanna and John Parks

Digital Credit: John Parks

Publisher: None

Rights: No rights reserved - image is in the public domain

Description: In vitro matured bovine oocytes have been denuded of most cumulus cells by vortexing. Denuding allows visualization of the ooplasm for such procedures as microinjection of nuclei for cloning, gene constructs for transfection, and addition and removal of cryoprotective agents (CPA) for cryopreservation. Presence of a single polar body in the perivitelline space indicates that oocytes have progressed to metaphase II.

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