Whole mount of an embryo on the left. On the right and in the middle are light micrographs stained with anti-Gata4 identified by the red color.  Sections were counterstained blue with hematoxylin. At this age, Gata4, a zinc finger transcription factor, is localized in the epithelium of the gut, in the hindgut dorsal mesentery cells (faint staining), and in coelomic epithelial cells that mark the position of the future gonad. Positive staining is absent in the mesonephros. McCoard et al., (Biol. Reprod. 65:366, 2001) published higher power images of Gata4 immunoreactive cells within the coelomic epithelium and hindgut dorsal mesentery.  The green arrows in the micrograph on the right illustrate the migration toward the coelomic epithelium of germ cells from the yolk sac and a subpopulation of somatic cells from the dorsal mesentery. These cells integrate into the developing gonad.

Porcine gonadogenesis - day 19 of gestation

Credit: Sue McCoard

Digital Credit: Sue McCoard

Publisher: USDA/ARS/U.S. Meat Animal Research Center

Rights: Image Gallery user terms

Description: Whole mount of an embryo on the left. On the right and in the middle are light micrographs stained with anti-Gata4 identified by the red color. Sections were counterstained blue with hematoxylin. At this age, Gata4, a zinc finger transcription factor, is localized in the epithelium of the gut, in the hindgut dorsal mesentery cells (faint staining), and in coelomic epithelial cells that mark the position of the future gonad. Positive staining is absent in the mesonephros. McCoard et al., (Biol. Reprod. 65:366, 2001) published higher power images of Gata4 immunoreactive cells within the coelomic epithelium and hindgut dorsal mesentery. The green arrows in the micrograph on the right illustrate the migration toward the coelomic epithelium of germ cells from the yolk sac and a subpopulation of somatic cells from the dorsal mesentery. These cells integrate into the developing gonad.

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